Fast, volumetric cell detection for multi-plane two-photon imaging

Paper

A citation will be added here once the paper is published.

Code availability

Suite3D is free software, released under the AGPL-3.0-or-later licence. The source, the demo notebooks and the analysis in this site are all in one repository:

https://github.com/alihaydaroglu/suite3d

pip install git+https://github.com/alihaydaroglu/suite3d.git

See the quickstart for the GPU requirements and for running a demo end to end.

Data availability

The three example recordings used by the demos are deposited on figshare. The demos do not download anything: unpack the archive and point --data-root at the folder.

https://rdr.ucl.ac.uk/articles/dataset/Volumetric_2-photon_imaging_datasets_used_to_test_Suite3D/32956220
DOI: 10.5522/04/32956220.v1

Dataset--data-root folderDownload
V1 v1/raw/*.tif 21.1 GB Mouse V1, 7 planes, standard resonant two-photon
LBM lbm/raw/*.tif 56.2 GB Light-bead microscopy, 22 planes
Hippocampus hippocampus/raw/*.tif 21.1 GB Dense, low-contrast, 4 planes

Every result on this site — the processing-steps panels, the ROI traces and the 3D viewers — is produced from these recordings by the pipeline in the repository above, at the parameters recorded in each demo's notebook.