Fast, volumetric cell detection for multi-plane two-photon imaging

Mouse V1

7 planes · 512×518 · standard resonant two-photon
Motion-corrected movie
Motion-corrected
Detection movie
Detection (mov_sub)
Correlation map
Correlation map
Scroll to zoom, drag to pan — all three panels move together. Click a cell to select it. Arrow keys step time (← →) and plane (↑ ↓); 0 resets the zoom.

Rigid motion

Rigid shift applied to each volume, in voxels. The marker follows the scrubber. Blocked non-rigid shifts are estimated on top of these and are not plotted.

Traces

Click a cell in any panel above.
Neuropil-corrected fluorescence (F − 0.7 × Fneu) and the deconvolved trace (spks), as written by the pipeline.
mov_sub is time-binned by the pipeline: one bin is 6 volumes. Both panels are averaged over the same window, so they show the same instant. One step of the time slider is 1.80 s, and the clip covers the first 108 s of the recording.