Fast, volumetric cell detection for multi-plane two-photon imaging

Hippocampus

4 planes · 514×512 · dense, low-contrast
Motion-corrected movie
Motion-corrected
Detection movie
Detection (mov_sub)
Correlation map
Correlation map
Scroll to zoom, drag to pan — all three panels move together. Click a cell to select it. Arrow keys step time (← →) and plane (↑ ↓); 0 resets the zoom.

Rigid motion

Rigid shift applied to each volume, in voxels. The marker follows the scrubber. Blocked non-rigid shifts are estimated on top of these and are not plotted.

Traces

Click a cell in any panel above.
Neuropil-corrected fluorescence (F − 0.7 × Fneu) and the deconvolved trace (spks), as written by the pipeline.
mov_sub is time-binned by the pipeline: one bin is 10 volumes. Both panels are averaged over the same window, so they show the same instant. One step of the time slider is 1.67 s, and the clip covers the first 100 s of the recording.